The effect of lemon extract (Citrus limon) on the blood sugar levels and pancreatic beta cell regeneration in alloxan-induced hyperglycemic mice

Petrus Mantur; Efrisca Meliyuita Br.  Damanik; Elisabeth Levina Sari  Setianingrum; Prisca Pakan

doi:10.32889/actabiona.97

Petrus Mantur Medicine Program, Faculty of Medicine and Veterinary Science, Universitas Nusa Cendana https://orcid.org/0000-0001-5262-5684
Efrisca Meliyuita Br. Damanik Department of Anatomical Pathology of Medicine Program, Faculty of Medicine and Veterinary Science, Universitas Nusa Cendana
Elisabeth Levina Sari Setianingrum Department of Clinical Pathology of Medicine Program, Faculty of Medicine and Veterinary Science, Universitas Nusa Cendana
Prisca Pakan Department of Microbiology of Medicine Program, Faculty of Medicine and Veterinary Science, Universitas Nusa Cendana

DOI: https://doi.org/10.32889/actabiona.97

Keywords: hyperglycemia, diabetes mellitus, lemon, alloxan

Abstract

Background: High blood sugar levels that exceed normal limits or commonly refered to as hyperglycemia, is an early symptom of diabetes mellitus.

Objectives: This study explored the effect of lemon (Citrus limon) extract on blood sugar levels and pancreatic b cell regeneration in alloxan-induced hyperglycemic mice (Mus musculus).

Method: This research is an experimental study using a post-test group. The sampling was carried out using a randomization method. By administering 125 mg/kg BW of alloxan, the sample was conditioned for hyperglycemia. The samples were divided into five groups: normal control, alloxan-induced control, and three treatment groups that received lemon extract with dosages of 100 mg/kg BW, 300 mg/kg BW and 500 mg/kg BW, respectively. Semiquantitative analysis was used to evaluate pancreatic damage.

Results: The results showed that lemon extract can decrease blood sugar levels. Histopathological imaging revealed a significant improvement in b cell distribution and decreased vacuolization in the Langerhans islets of mice administered lemon extract. No significant differences were observed among different dosages of lemon extract (p>0.05).

Conclusion: Our findings underscore the potential therapeutic benefits of lemon extract in managing blood sugar levels and promoting pancreatic b cell regeneration in alloxan-induced mice.

References

Magliano D, Boyko E. IDF Diabetes Atlas 2021. vol. 10. 2021.

Otto-Buczkowska E, Pilśniak A. Can Type 2 Diabetes Be Cured? 2020;15:304-10.

Kemenkes RI. Hari Diabetes Sedunia Tahun 2018. Pus Data Dan Inf Kementrian Kesehat RI 2018:1-8.

Sihotang RC, Ramadhani R, Tahapary DL. Efikasi dan Keamanan Obat Anti Diabetik Oral pada Pasien Diabetes Melitus Tipe 2 dengan Penyakit Ginjal Kronik. J Penyakit Dalam Indones 2018;5:150. https://doi.org/10.7454/jpdi.v5i3.202

Pakan P, Lidia K, Riwu M. Investigation of ginger (Zingiber officinale) aqueous extract as an anti-diabetic in vitro. IOP Conf Ser Earth Environ Sci 2021;913. https://doi.org/10.1088/1755-1315/913/1/012108

Derbin Vijay VP, Praveen D, Ranadheer Chowdary P, Vijey Aanandhi M. Metformin-induced lactic acidosis: A review. Drug Invent Today 2018;10:216-8.

Rajendiran D, Packirisamy S, Gunasekaran K. A review on role of antioxidants in diabetes. Asian J Pharm Clin Res 2018;11:48-53. https://doi.org/10.22159/ajpcr.2018.v11i2.23241

Qudah T, Rehman R, Sadique S, Nisar S, Al-Qudah TS, Zahra U, et al. Lemon as a source of functional and medicinal ingredient: A review Medicinal essential oils from plants of cypress family View project Medicinal plants View project Lemon as a source of functional and medicinal ingredient: A review. Ijcbs 2018;14:55.

Lindawati NY, Nofitasari J. Efektivitas Sari Buah Lemon (Citrus limon (L.) Burm. f. sebagai Khelating Agent Logam Berat Tembaga. J Farm Dan Ilmu Kefarmasian Indones 2021;8:68. https://doi.org/10.20473/jfiki.v8i12021.68-73

Permatasari R, Andriane Y, Garna H, Haribudiman O, Ekowati RAR. Pengaruh Fraksi Air Buah Lemon (Citrus limon) terhadap Kadar Glukosa Darah Mencit Tua yang Diberi Pakan Tinggi Lemak. J Integr Kesehat Sains 2019;1:54-8. https://doi.org/10.29313/jiks.v1i1.4322

Neovita E, Solihah PSD, Wahyuningsih S, Aeni HH, Azhari F. Pengembangan Ekstrak Etanol Kulit Jeruk Lemon (Citrus limon (L.)) sebagai Antidiabetes Oral. Kartika J Ilm Farm 2021;8:1. https://doi.org/10.26874/kjif.v8i1.220

Kadhum RJ, Obaid QA, Ahmed AJ, Hussein AM. Comparative study of physiological effect of lemon juice and aqueous extract of pumpkin fruits on diabetic female mice. Plant Arch 2020;20:922-6.

El-esawy BH, Askary A El, Elmorsy E. Histopathological Evaluation of the Pancreas Following Administration of Paricalcitol in Alloxan- Induced Diabetic Wistar Rats 2016;5:189-98.

Ravif F. Gambaran Histologi Organ Hepar, Pankreas, dan Ginjal Tikus Jantan Strain Sprague Dawley dengan Teknik Perfusi PBS. 2016.

Yasaroh S, Christijanti W, Lisdiana, Iswari, S R. Efek ekstrak daun kelor (Moringa oleifera) terhadap kadar glokosa darah tikus diabetes induksi aloksan. Pros Semnas Biol Ke-9 Tahun 2021 FMIPA Univ Negeri Semarang 55 2021:224-9.

Ighodaro OM, Adeosun AM, Akinloye OA. Alloxan-induced diabetes, a common model for evaluating the glycemic-control potential of therapeutic compounds and plants extracts in experimental studies. Med 2017;53:365-74. https://doi.org/10.1016/j.medici.2018.02.001

Kilimnik G, Jo J, Periwal V, Zielinski MC, Hara M. Quantification of islet size and architecture. Islets 2012;4:167-72. https://doi.org/10.4161/isl.19256

Bosco D, Armanet M, Morel P, Niclauss N, Sgroi A, Muller YD, et al. Unique arrangement of alpha- and beta-cells in human islets of Langerhans. Diabetes 2010;59:1202-10. https://doi.org/10.2337/db09-1177

Coppieters KT, von Herrath MG. Histopathology of type 1 diabetes: old paradigms and new insights. Rev Diabet Stud 2009;6:85-96. https://doi.org/10.1900/RDS.2009.6.85

Shubin A V, Demidyuk I V, Komissarov AA, Rafieva LM, Kostrov S V. Cytoplasmic vacuolization in cell death and survival. Oncotarget 2016;7:55863-89. https://doi.org/10.18632/oncotarget.10150