Cytotoxic activity and selectivity index of binahong (Anredera cordifolia) extracts on MCF-7 breast cancer cells and vero normal cells line
Abstract
Background: Breast cancers occur because of an impaired balance between proliferation, differentiation, and apoptosis of breast glands. Natural products have potency as cytotoxic agents with less side effects than chemotherapy. One of the potential plants is Anredera cordifolia (Ten.) Stennis (binahong), which contains flavonoid 8-glucopyranosyl- 4’,5,7-trihydroxy flavone compounds.
Objective: This study aims to determine the potency of binahong leaves extract as an anticancer for breast cancer in vitro and in silico.
Methods: Preliminary tests using molecular docking of 8-glucopyranosyl-4’,5,7-trihydroxyflavone compounds on Bcl-2 and HER-2 proteins. The extraction and fractionation were to obtain binahong extract. Thin layer chromatography to identify flavonoid compounds in the extract. DPPH assay was performed to evaluate the antioxidant activity. MTT assay was performed to evaluate cytotoxic activity on MCF-7 breast cancer cells and Vero cells.
Results: In silico test showed a stable bond between 8-glucopyranosyl- 4’,5,7-trihydroxylflavone, and Bcl-2 and HER-2 with a docking score of -7.5 kcal/mol and -8.0 kcal/mol, respectively. The binahong extract contain flavonoid compounds that had the retention factor value 0.78; 0.49; 0.35. Antioxidant test resulted IC50 value of 4940 μg/mL. Cytotoxic test resulted in IC50 value of 1073 μg/mL and 486 μg/mL for Vero cells and MCF-7 breast cancer cells, respectively. The comparison between IC50 produced a selectivity index value of 2.149, which shows that binahong extract was selective against MCF-7 breast cancers.
Conclusion: This study concluded that binahong extract has weak potency as anticancer agent on MCF-7 cells.
References
GLOBOCAN [internet]. Indonesia - Global Cancer Observatory [cited 2020 Des 15]. Available from : https://gco.iarc.fr/today/data/factsheets/populations/360-indonesia-fact-sheets.pdf
Nita YH, Ana M, Supriatno. Potensi daya hambat ekstrak etanol daun binahong (Anredera cordifolia (ten.) steenis) terhadap proliferasi sel kanker lidah manusia (SP-C1) in vitro. Yogyakarta: Universitas Muhammadiyah Yogyakarta. 2013.
Ratna D, Wahyudi PS, Wahono S, Hanafi. Antioxidant activity of flavonoid from Anredera cordifolia (ten) steenis leaves. International Research Journal of Pharmacy. 2012;3(9):241-243.
Quiroga R, Villarreal MA. Vinardo: A scoring function based on autodock vina improves scoring, docking, and virtual screening. PLoS ONE. 2016;11(5):e0155183. https://doi.org/10.1371/journal.pone.0155183
Abubakar AR, Haque M. Preparation of medicinal plants: Basic extraction and fractionation procedures for experimental purposes. J Pharm Bioall. Sci. 2020 ;12:1-10. https://doi.org/10.4103/jpbs.JPBS_175_19
Li C. Thin layer chromatography. Current Protocols Essential Laboratory Techniques. 2014; https://doi.org/10.1002/9780470089941.et0603s08
Marxen K, Vanselow KH, Lippemeier S, Hintze R, Ruser A, Hansen VP. Determination of DPPH radical oxidation caused by methanolic extracts of some microalgae species by linear regression analysis of spectrophotometric measurements. Sensors. 2007;7:2080-95. https://doi.org/10.3390/s7102080
Tailor CS, Goyal A. Antioxidant activity by DPPH radical scavenging method of Ageratum conyzoides Linn. leaves. American Journal of Ethnomedicine. 2014;1(4):244-249.
Cree, Ian A. Cancer cell culture : Methods and protocols. 2nd ed. New York: Humana Press;2011. Print.
Dedic, Natalija P, Ana K, Damir V. Role of HER2 signaling pathway in breast cancer: Biology, stection and therapeutical implications. Periodicum Biologorium. 2012;114(4):505-510.
Abotable, Mariam, Samson MS, Elizabeth V. Flavonoids in cancer and apoptosis. Cancers. 2019:11-28. https://doi.org/10.3390/cancers11010028
Harborne JB. Phytochemical methods: A guide to modern technique of plant analysis. 2nd ed. London; Chapman and Hall;1984.19:37-168.
Robinson T. Kandungan organik tumbuhan tinggi (Translated by Kosasih Padmawinata). Edisi IV. Bandung: ITB; 1995. 191-216.
Gibbons S. An Introduction to planar chromatography. Totowa New Jersey: Human Press; 2006. https://doi.org/10.1385/1-59259-955-9:77
Gandjar IG, Rohman A. Kimia Farmasi Analisis. Yogyakarta: Pustaka Pelajar; 2012.
Budilaksono W, Wahdaningsih S, Fahruroji A. Uji aktivitas antioksidan fraksi N-heksana kulit buah naga merah (Hylocereus Iemarirei Britton dan Rose) menggunakan metode DPPH. Jurnal Mahasiswa Farmasi Fakultas Kedokteran UNTAN. 2014;1(1).
Mardawati EF, Filianty, Marta H. Kajian aktivitas antioksidan ekstrak kulit manggis (Garcinia mangostana L) dalam rangka pemanfaatan limbah kulit manggis di kecamatan puspahiang kabupaten tasikmalaya [Skripsi]. Bandung: Universitas Padjadjaran; 2008.
Weerapreeyakul N, Nonpunya A, Barusrux S, Thitimetharoch T, Sripanidkulchi B. Evaluation of the anticancer potential of six herbs against a hepatoma cell line. Chinese Medicine. 2012:7;1-7. https://doi.org/10.1186/1749-8546-7-15
Prayong P, Barusrux S, Weerapreeyakul N. Cytotoxic activity screening of some indigenous Thai plants. Fitoterapia. 2008;79(7-8):598-601. https://doi.org/10.1016/j.fitote.2008.06.007
Badisa RB, Darling-Reed SF, Joseph P, Cooperwood JS, Latinwo LM. Selective cytotoxic activities of two novelsynthetic drugs on human breast carcinoma MCF7 Cells. Anticancer Res. 2009;29:2993-2996
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