A study on the hepatoprotective effect of turmeric (Curcuma longa) extract on the liver histopathology of albino rats induced by sodium diclofenac
Abstract
Background: Diclofenac sodium, a widely used nonsteroidal anti-inflammatory drug (NSAID), causes significant hepatotoxicity through oxidative stress mechanisms. Turmeric (Curcuma longa L.), rich in curcuminoid antioxidants, may offer hepatoprotection.
Objective: To evaluate the hepatoprotective effects of turmeric extract against diclofenac sodium-induced liver injury in rats.
Methods: Twenty-eight male Sprague Dawley rats were randomly divided into four groups (n=7): normal control, negative control (diclofenac sodium 10 mg/kg BW for 7 days), and two treatment groups receiving diclofenac sodium followed by turmeric extract at 100 or 200 mg/kg BW for 14 days. Liver histopathology was assessed using hematoxylin-eosin staining. Data were analyzed descriptively.
Results: Turmeric extract attenuated hepatocellular damage in a dose-dependent manner. The 200 mg/kg BW dose completely prevented necrosis, demonstrating superior hepatoprotection compared to 100 mg/kg BW.
Conclusion: Turmeric extract exerts hepatoprotective effects against diclofenac-induced liver injury through attenuation of histopathological damage.
References
Mardhiyah R, Fauzi A, Syam AF. Diagnosis dan Tata Laksana Enteropati akibat Obat Anti Inflamasi Non Steroid (OAINS). J Penyakit Dalam Indones. 2017;2: 190. https://doi.org/10.7454/jpdi.v2i3.84
Mangampa I, Nugroho TE. Pengaruh Pemberian Natrium Diklofenak Dosis 1,4 Mg/kgbb Dan 2,8 Mg/kgbb Terhadap Kadar Serum Kreatinin Tikus Wistar. Jurnal Kedokteran Diponegoro. 2015;4: 1004-1012.
Meunier L, Larrey D. Recent advances in hepatotoxicity of non steroidal anti-inflammatory drugs. Ann Hepatol. 2018;17: 187-191. https://doi.org/10.5604/01.3001.0010.8633
Soleha M, Isnawati A, Fitri N, Adelina R, Soblia HT, Winarsih W. Profil Penggunaan Obat Antiinflamasi Nonstreoid di Indonesia. J Kefarmasian Indones. 2018; 109-117. https://doi.org/10.22435/jki.v8i2.316
Bischoff K, Mukai M, Ramaiah SK. Liver toxicity. Veterinary Toxicology. Elsevier; 2018. pp. 239-257. https://doi.org/10.1016/B978-0-12-811410-0.00015-5
Sobhani K, Li J, Cortes M. Nonsteroidal anti-inflammatory drugs (NSAIDs). First Aid Perioperative Ultrasound. Cham: Springer International Publishing; 2023. pp. 127-138. https://doi.org/10.1007/978-3-031-21291-8_8
Hoofnagle JH. LiverTox. Drug-Induced Liver Disease. Elsevier; 2013. pp. 725-732. doi:10.1016/B978-0-12-387817-5.00040-6. https://doi.org/10.1016/B978-0-12-387817-5.00040-6
Jung S-H, Lee W, Park S-H, Lee K-Y, Choi Y-J, Choi S, et al. Diclofenac impairs autophagic flux via oxidative stress and lysosomal dysfunction: Implications for hepatotoxicity. Redox Biol. 2020;37: 101751. https://doi.org/10.1016/j.redox.2020.101751
Owumi SE, Dim UJ. Biochemical alterations in diclofenac-treated rats: Effect of selenium on oxidative stress, inflammation, and hematological changes. Toxicol Res Appl. 2019;3: 239784731987435. https://doi.org/10.1177/2397847319874359
Palawe CY, Kairupan CF, Lintong PM. Efek Hepatoprotektif Tanaman Obat. Medical Scope Journal. 2021;3: 61. https://doi.org/10.35790/msj.v3i1.33542
Munadi E. Info Komoditi Tanaman Obat: Bab I Tanaman Obat, Sebuah Tinjauan Singkat. Jakarta: Badan Pengkajian dan Pengembangan Perdagangan Kementerian Perdagangan Republik Indonesia. 2017.
Munaeni W, Mainassy MC, Puspitasari D, Susanti L and Endriyanto NC, Yuniastuti A. Perkembangan dan Manfaat Obat Herbal sebagai Fitoterapi. Tohar Media; 2017.
Septiana E, Simanjuntak P. Aktivitas antimikroba dan antioksidan ekstrak beberapa bagian tanaman kunyit (Curcuma longa). FITOFARMAKA: Jurnal Ilmiah Farmasi. 2015;5: 1-10. https://doi.org/10.33751/jf.v5i1.193
Utami ET, Aqlina DS, Fajariyah S, Mahriani M. Pengaruh Pemberian Ekstrak Rimpang Kunyit (Curcuma longa L.) terhadap Histologi Hepar Tikus Pasca Diinduksi Thioacetamide (TAA). Bioscientist j ilm bosbiol. 2022;10: 950. https://doi.org/10.33394/bioscientist.v10i2.6011
M Thuawaini M, Al-Farhaan MBG, F Abbas K. Hepatoprotective and nephroprotective effects of the aqueous extract of turmeric (curcuma longa) in rifampicin and isoniazid-induced hepatotoxicity and nephrotoxicity in rats. Asian J Pharm Clin Res. 2019; 293-298. https://doi.org/10.22159/ajpcr.2019.v12i3.30419
Yana EF, Budijastuti W. Gambaran Histopatologi Toksisitas Hepar Tikus Jantan (Rattus norvegicus) Pasca Pemberian Sirup Umbi Yakon (Smallanthus sonchifolius). LenteraBio: Berk Ilm Biol. 2022;11: 202-207. https://doi.org/10.26740/lenterabio.v11n1.p202-207
Maulina M. Zat-zat yang Mempengaruhi Histopatologi Hepar. Aceh: Unimal Press; 2018.
Kumar V, Abbas AK, Aster JC. Buku Ajar Patologi Dasar Robbins. Chicago: Elsevier Health Sciences; 2019.
Esperanza Y, Prabowo S, Handajani F. Pengaruh Pemberian Curcumin Terhadap Perbaikan Fungsi Hepar Tikus Putih (Rattus Novergicus) yang diinduksi Parasetamol Dosis Tinggi. JIlmKedokt Wijaya Kusuma. 2021;10: 208. https://doi.org/10.30742/jikw.v10i2.1250
El-Maddawy ZK, El-Sayed YS. Comparative analysis of the protective effects of curcumin and N-acetyl cysteine against paracetamol-induced hepatic, renal, and testicular toxicity in Wistar rats. Environmental Science and Pollution Research. 2018;25: 3468-3479. https://doi.org/10.1007/s11356-017-0750-3
Sulasiyah S, Sarjono PR, Aminin ALN. Antioxidant from Turmeric Fermentation Products (Curcuma longa) by Aspergillus Oryzae. J Kim Sains Dan Apl. 2018;21: 13-18. https://doi.org/10.14710/jksa.21.1.13-18
Copyright (c) 2025 Authors
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
