Dose-dependent nephroprotective effects of turmeric extract against diclofenac-induced kidney injury in rats
Abstract
Background: Non-steroidal anti-inflammatory drugs, particularly diclofenac sodium, are widely prescribed but can cause nephrotoxicity through oxidative stress mechanisms. Turmeric (Curcuma longa L.) contains curcumin and other bioactive compounds with potent antioxidant properties that may protect against drug-induced kidney damage.
Objective: To evaluate the dose-dependent effects of turmeric ethanol extract on kidney histopathology in rats with established diclofenac sodium-induced nephrotoxicity.
Methods: Twenty-eight male Sprague Dawley rats were randomly divided into four groups: normal control, diclofenac (10 mg/kg for 7 days), and two treatment groups receiving diclofenac followed by turmeric extract at 100 mg/kg or 200 mg/kg for 14 days. Kidney histopathology was assessed using the Arsad scoring system by a blinded pathologist.
Results: Significant differences existed between groups (p < 0.001). The negative control exhibited severe kidney damage with hydropic degeneration, granular casts, and cellular casts (mean score: 2.78 ± 0.24). Treatment with 100 mg/kg showed partial improvement (1.65 ± 0.31), while 200 mg/kg demonstrated substantial improvement approaching normal histology (0.52 ± 0.18) with only minimal residual damage.
Conclusion: Turmeric extract demonstrates significant dose-dependent nephroprotective effects against diclofenac-induced kidney damage, with 200 mg/kg providing superior protection, suggesting potential therapeutic applications in mitigating NSAID-induced nephrotoxicity.
References
Doğruyol S, Akbaş .Ilker, Koçak AO, Aygörmez S, Leylek Habip Emrah and Akgöl Gür ST, Ertener Ö. Can spesific biomarkers be used to enlighten the major mechanisms of acute high dose diclofenac sodium-related nephrotoxicity? Eurasian J Emerg Med. 2022;21: 132-137. https://doi.org/10.4274/eajem.galenos.2021.45467
Albassit S, Al Laham S, Al-Manadili A. Histological assessment of Selenium protective effect against Diclofenac Sodium-induced nephrotoxicity in rats. J Pharm Nutr Sci. 2023;13: 1-12. https://doi.org/10.29169/1927-5951.2023.13.01
Raeeszadeh M, Ahmadi Simab P. Ameliorative effect of methanolic extract of broccoli (BMX) on diclofenac sodium (DIC)-induced oxidative damage in rat kidney. J Lab Anim Res. 2022;1: 14-19. https://doi.org/10.58803/jlar.v1i1.4
Abiola TS, Adebayo OC, Babalola OO. Diclofenac-induced kidney damage in wistar rats: Involvement of antioxidant mechanism. J Biosci Med (Irvine). 2019;07: 44-57. https://doi.org/10.4236/jbm.2019.712005
Vyas T. Role of curcumin as an antioxidant. J Prim Care Dent Oral Health. 2020;1: 8. https://doi.org/10.4103/jpcdoh.jpcdoh_1_20
Wardhani FM, Chiuman L, Ginting CN, Ginting SF, Nasution AN. Efek Ekstrak Kunyit Putih (Curcuma Zedoaria) Sebagai Nefroprotektor Pada Tikus Putih Jantan Galur Wistar yang Diinduksi Tembaga. J Indon Med Assoc. 2020;69: 258-266. https://doi.org/10.47830/jinma-vol.69.8-2019-186
Chanda STR. Phytochemical and pharmacological importance of turmeric. American Journal of Pharmacology. 2019;9: 16-23.
Ragab SMM, Abd-Elkareem M, Abou Khalil NS, Atia MM. Protective effect of curcumin on the kidney of diclofenac sodium-challenged mice: apoptotic, redox potential and histopathological outcomes. J Basic Appl Zool. 2022;83. https://doi.org/10.1186/s41936-022-00315-5
Owumi SE, Dim UJ. Biochemical alterations in diclofenac-treated rats: Effect of selenium on oxidative stress, inflammation, and hematological changes. Toxicol Res Appl. 2019;3: 239784731987435. https://doi.org/10.1177/2397847319874359
M Thuawaini M, Al-Farhaan MBG, F Abbas K. Hepatoprotective and nephroprotective effects of the aqueous extract of turmeric (curcuma longa) in rifampicin and isoniazid-induced hepatotoxicity and nephrotoxicity in rats. Asian J Pharm Clin Res. 2019; 293-298. https://doi.org/10.22159/ajpcr.2019.v12i3.30419
Zhao Y-H, Shen C-F, Wang G-J, Kang Yan and Song Y-H, Liu J-W. Curcumin alleviates acute kidney injury in a dry-heat environment by reducing oxidative stress and inflammation in a rat model. J Biochem Mol Toxicol. 2021;35: e22630. https://doi.org/10.1002/jbt.22630
Arsad SS. Histopathologic Changes in Liver and Kidney Tissues from Male Sprague Dawley Rats Treated with Rhaphidophora Decursiva (Roxb.) Schott Extract. J Cytol Histol. 2014;s4. https://doi.org/10.4172/2157-7099.S4-001
Mousa AA, Elweza AE, Elbaz HT, Tahoun EAE-A, Shoghy KM, Elsayed I, et al. Eucalyptus Globulus protects against diclofenac sodium induced hepatorenal and testicular toxicity in male rats. J Tradit Complement Med. 2020;10: 521-528. https://doi.org/10.1016/j.jtcme.2019.11.002
Mustafa HN, Alkan I, Deniz ÖG, Altunkaynak BZ, Annaç E, Kaplan S. A study on the toxic effect of different doses of diclofenac sodium on the development of the kidney in the postnatal period. Int J Morphol. 2019;37: 877-884. https://doi.org/10.4067/S0717-95022019000300877
Qasim LB, Jasim GA, Rabeea IS. Histopathological study of diclofenac induced acute renal failure under lipoic acid and bosentan therapy in male albino rats. Al Mustansiriyah Journal of Pharmaceutical Sciences. 2022;22: 49-58. https://doi.org/10.32947/ajps.v22i1.829
Rajesham V V, Kandoti HS, Raghavendra Mitta and Ali PR, Rao TR. Nephroprotective activity of ethanolic extract of Carissa carandas leaves against Gentamicin-induced acute kidney injury in Wistar albino rats. J Pharm Res Int. 2021; 279-286. https://doi.org/10.9734/jpri/2021/v33i58A34117
Mba O, Emeka IE, Peter IC, Oghenesuwe EE, Osaremen AJ, Nnenna A. Nephroprotective Effects of Balanites aegyptiaca (l.) delile Stem-Bark Extract and Fractions against Diclofenac-Induced Nephrotoxicity in Rats. Tropical Journal of Natural Product Research. 2021;5: 1300-1308. https://doi.org/10.26538/tjnpr/v5i7.23
Tandi J, Melinda B, Purwantari A, Widodo A. Analisis Kualitatif dan Kuantitatif Metabolit Sekunder Ekstrak Etanol Buah Okra (Abelmoschus esculentus L. Moench) dengan Metode Spektrofotometri UV-Vis. KOVALEN. 2020;6: 74-80. https://doi.org/10.22487/kovalen.2020.v6.i1.15044
Nola F, Putri GK, Malik Lhidya Halizah and Andriani N. Isolasi Senyawa Metabolit Sekunder Steroid dan Terpenoid dari 5 Tanaman. Syntax Idea. 2021;3: 1612-1619. https://doi.org/10.46799/syntax-idea.v3i7.1307
Konak Ş, Şener EH. Antioxidant effects of curcumin on the blood tissue in rats. Mehmet Akif Ersoy Üniv Sağlk Bilim Enst derg. 2019;7: 8-14. https://doi.org/10.24998/maeusabed.539891
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