The effect of fermented tempeh aerobic anaerobic (FETAA) on pancreatic duodenal homeobox 1 (Pdx1) gene expression and HOMA-beta index in diabetic mice

Hendi Wicaksono; Prasetyastuti Prasetyastuti; Pramudji Hastuti; Ahmad Hamim Sadewa

doi:10.32889/actabioina.11

Hendi Wicaksono Duta Wacana Christian University, Jl. Dr Wahidin Sudirohusodo No 5- 25, Gondokusuman, Yogyakarta, Indonesia
Prasetyastuti Prasetyastuti Department of Biochemistry Faculty of Medicine, Public Health and Nursing, Gadjah Mada University, Jl. Farmako, Depok, Sleman, Yogyakarta, Indonesia
Pramudji Hastuti Department of Biochemistry Faculty of Medicine, Public Health and Nursing, Gadjah Mada University, Jl. Farmako, Depok, Sleman, Yogyakarta, Indonesia
Ahmad Hamim Sadewa Department of Biochemistry Faculty of Medicine, Public Health and Nursing, Gadjah Mada University, Jl. Farmako, Depok, Sleman, Yogyakarta, Indonesia

DOI: https://doi.org/10.32889/actabioina.11

Keywords: gamma aminobutyric acid, HOMA-beta, pancreatic duodenal homeobox 1, type 2 diabetes mellitus, fermented tempeh aerobic anaerobic

Abstract

Background: Diabetes is a result of oxidative stress which causes the impaired function of pancreatic beta-cells. Fermented tempeh aerobic anaerobic (FETAA) containing gamma-aminobutyric acid and isoflavones can reduce oxidative stress in diabetes.

Objective: The aim of this study is to evaluate FETAA in improving pancreatic β-cell function in diabetic mice.

Methods: Twenty streptozotocin-induced diabetic mice, divided into four groups (n = 5 each group): DM, DM + FETAA 10 mg/100 g BW, DM + FETAA 20 mg/100 g BW, DM + FETAA 40 mg/100 g BW as well as normal group (n = 5). DM mice were treated with FETAA for 21 days. Fasting glucose was determined using the GOD-PAP method, while insulin level was determined by ELISA. The homeostasis model assessment of β-cell function (HOMA-β) was calculated using the HOMA2 calculator, and the Pdx1 mRNA level was determined by Real Time-PCR.

Results: The DM mice group treated with FETAA had lower glucose levels than the DM mice group. FETAA dosage of 40 mg/100 g BW was able to reduce the highest blood glucose levels (p<0.05). DM mice group treated with FETAA had higher levels of insulin and HOMA-β than the DM mice group (p <0.05). Treatment of FETAA 10 mg/100 g BW produced the highest insulin content of 57.44 ± 8.132 pmol/L, while treatment of FETAA 40 mg/100 g BW had a HOMA-β value of 72.86 ± 21.85%. Pdx1 mRNA expression in group FETAA-treated DM mice was higher than the DM mice group, although it was not statistically significant (p> 0.05).

Conclusion: FETAA could improve HOMA-β, blood glucose levels, but did not affect Pdx1 mRNA expression.

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