Ethanol extract of Bruguiera gymnorrhiza mangrove leaves and propolis activity on macroscopic healing of cuts in vivo

Evi Kurniawaty; Shina Megaputri; Syazili Mustofa; Soraya Rahmanisa; Kholis Abdurachim Audah; Silvia Andriani

doi:10.32889/actabioina.94

Evi Kurniawaty Department of Biochemistry and Biomolecular, Faculty of Medicine, University of Lampung, Lampung, 35141, Indonesia https://orcid.org/0000-0003-0610-5957
Shina Megaputri Department of Biochemistry and Biomolecular, Faculty of Medicine, University of Lampung, Lampung, 35141, Indonesia
Syazili Mustofa Department of Biochemistry and Biomolecular, Faculty of Medicine, University of Lampung, Lampung, 35141, Indonesia https://orcid.org/0000-0002-7646-0869
Soraya Rahmanisa Department of Biochemistry and Biomolecular, Faculty of Medicine, University of Lampung, Lampung, 35141, Indonesia
Kholis Abdurachim Audah Department of Biomedical Engineering, Faculty of Life Sciences and Technology, Swiss German University, Tangerang, Indonesia https://orcid.org/0000-0003-1105-6387
Silvia Andriani Departement of Biology, Faculty of Mathematics and Natural Sciences, University of Lampung, 35141, Indonesia https://orcid.org/0000-0002-8630-1714

DOI: https://doi.org/10.32889/actabioina.94

Keywords: Bruguiera gymnorrhiza, propolis, wound healing

Abstract

Background: Bruguiera gymnorrhiza mangrove and propolis are often used as natural wound treatments. Its constituent is believed to promote wound healing.

Objective: This study aims to explore the effect of topical administration of ethanol extract of Bruguiera gymnorrhiza mangrove leaves and propolis on wound healing activity.

Methods: Twenty-four Sprague Dawley rats were randomly assigned to one of four treatment groups (n=6): aqua dest, standard wound medicine, ethanol extract of Bruguiera gymnorrhiza mangrove leaves, and propolis extract. Following the incision, treatment was given once a day for 14 days or until the wound healed. The cut area was observed by measuring the cut length using a ruler.

Results: Standard wound medication took 9 days to promote healing, while the negative control (aqua dest) took 11.2 days, the ethanol extract of Bruguiera gymnorrhiza mangrove leaves took 7.3 days, and the propolis extract took 7.9 days.

Conclusions: Topical administration of Bruguiera gymnorrhiza mangrove leaves ethanol extract and propolis had equal effects on the healing of wounds. Both are more effective than conventional wound ointments at healing cuts.

References

Kim JY, Dao H. Physiology, Integument. StatPearls. Treasure Island (FL): StatPearls Publishing; 2022.

Kalangi SJ. Histofisiologi kulit. JBM. 2014;5. https://doi.org/10.35790/jbm.5.3.2013.4344

Nurmalasari Y, Nofita N, Warganegara E, Sijabat AI. Perbandingan air perasan daucus carota L. dengan povidone iodine topikal dalam penyembuhan luka insisi mencit. 2020;12: 673-679. https://doi.org/10.35816/jiskh.v12i2.378

Lotfollahzadeh S, Burns B. Penetrating Abdominal Trauma. StatPearls. Treasure Island (FL): StatPearls Publishing; 2022.

Halim E, Hardinsyah H, Sutandyo N, Sulaeman A, Artika M, Harahap Y. Kajian bioaktif dan zat gizi propolis indonesia dan brasil. jgizipangan. 2013;7: 1. https://doi.org/10.25182/jgp.2012.7.1.1-7

Sur TK, Hazra A, Hazra AK, Bhattacharyya D. Antioxidant and hepatoprotective properties of Indian Sunderban mangrove Bruguiera gymnorrhiza L. leave. J Basic Clin Pharm. 2016;7: 75-79. https://doi.org/10.4103/0976-0105.183262

Syahidah, Subekti N. Phytochemical Analysis of Mangrove Leaves (Rhizophora sp.). IOP Conf Ser: Mater Sci Eng. 2019;593: 012007. https://doi.org/10.1088/1757-899X/593/1/012007

Wagh VD. Propolis: a wonder bees product and its pharmacological potentials. Adv Pharmacol Sci. 2013;2013: 308249. https://doi.org/10.1155/2013/308249

Coutinho A. Honeybee propolis extract in periodontal treatment: a clinical and microbiological study of propolis in periodontal treatment. Indian J Dent Res. 2012;23: 294. https://doi.org/10.4103/0970-9290.100449

Cornara L, Biagi M, Xiao J, Burlando B. Therapeutic Properties of Bioactive Compounds from Different Honeybee Products. Front Pharmacol. 2017;8: 412. https://doi.org/10.3389/fphar.2017.00412

Salatino A, Salatino MLF, Negri G. How diverse is the chemistry and plant origin of Brazilian propolis? Apidologie. 2021;52: 1075-1097. https://doi.org/10.1007/s13592-021-00889-z

Bioplacenton KalbeMed [Internet]. [cited 20 Jul 2022]. Available: https://kalbemed.com/product/id/101

Anggraini RR, Hendri M, Rozirwan R. Potensi larutan bubuk daun mangrove Bruguiera gymnorrhiza sebagai pengawet alami. Maspari Journal : Marine Science Research. 2018;10: 51-62.

Khorasgani EM, Karimi AH, Nazem MR. A Comparison of Healing Effects of Propolis and Silver Sulfadiazine on Full Thickness Skin Wounds in Rats. Pakistan Veterinary Journal. 2010;30: 72-74.

Khairunnisa B, Rosamah E, Kuspradini H, Kusuma IW, Sukemi S, Tandirogang N, et al. Uji fitokimia dan antioksidan ekstrak etanol propolis lebah kelulut (Tetragonula iridipennis) dari Samarinda Kalimatan Timur. Jurnal Ilmiah Manuntung. 2020;

Chittasupho C, Manthaisong A, Okonogi S, Tadtong S, Samee W. Effects of quercetin and curcumin combination on antibacterial, antioxidant, in vitro wound healing and migration of human dermal fibroblast cells. Int J Mol Sci. 2021;23. https://doi.org/10.3390/ijms23010142

Fernandez O, Capdevila JZ, Dalla G, Melchor G. Efficacy of Rhizophora mangle aqueous bark extract in the healing of open surgical wounds. Fitoterapia. 2002;73: 564-568. https://doi.org/10.1016/S0367-326X(02)00229-0

Ibrahim N 'Izzah, Wong SK, Mohamed IN, Mohamed N, Chin K-Y, Ima-Nirwana S, et al. Wound healing properties of selected natural products. Int J Environ Res Public Health. 2018;15. https://doi.org/10.3390/ijerph15112360

Liu E, Gao H, Zhao Y, Pang Y, Yao Y, Yang Z, et al. The potential application of natural products in cutaneous wound healing: A review of preclinical evidence. Front Pharmacol. 2022;13. https://doi.org/10.3389/fphar.2022.900439