Estimation of malondialdehyde and catalase activity in pregnant women at IIMS&R Hospital, Lucknow, India

Niharika Singh; Saba Khan; Mohammad Mustufa Khan; Haseeb Ahsan; Roshan Alam

doi:10.32889/actabioina.89

Niharika Singh Department of Biochemistry, Integral Institute of Medical Sciences & Research (IIMS&R), Integral University, Lucknow, UP, India.
Saba Khan Department of Biochemistry, Integral Institute of Medical Sciences & Research (IIMS&R), Integral University, Lucknow, UP, India. https://orcid.org/0000-0002-6140-8152
Mohammad Mustufa Khan Department of Basic Medical Sciences, Integral Institute of Allied Health Sciences & Research (IIAHS&R), Integral University, Lucknow, UP, India
Haseeb Ahsan Department of Biochemistry, Faculty of Dentistry, Jamia Millia Islamia, Jamia Nagar, New Delhi, India https://orcid.org/0000-0002-5313-5959
Roshan Alam Department of Biochemistry, Integral Institute of Medical Sciences & Research (IIMS&R), Integral University, Lucknow, UP, India. https://orcid.org/0000-0003-1944-8924

DOI: https://doi.org/10.32889/actabioina.89

Keywords: catalase, oxidative stress, malondialdehyde, pregnancy, reactive oxygen species

Abstract

Background: During pregnancy, the physiological production of reactive oxygen species (ROS) is associated with a variety of maternal, placental, and fetal developmental functions. These functions are disrupted by excessive amounts of ROS, resulting to pregnancy complications. Different stages of pregnancy require a balance between oxidant and antioxidant production.

Objective: The aim of this study is to investigate the status of malondialdehyde (MDA) and catalase (CAT) activity in pregnant and age matched non-pregnant women.

Methods: In this cross-sectional study, a total of 74 participants were enrolled, including 37 pregnant and 37 age-matched non-pregnant women. The age range of participants was 18 to 40 years. MDA and CAT levels were measured spectrophotometrically. A p-value 0.05 was statistically significant.

Results: Compared to non-pregnant women, the mean plasma level of MDA was considerably elevated in pregnant women (p<0.0001). However, the mean level of CAT activity in pregnant women was significantly lower than in non-pregnant women (p<0.0001) In pregnant women, there is a significant strong negative correlation between MDA levels and CAT activity (p<0.01).

Conclusion: Pregnant women have high level of oxidative stress, indicating that the pregnant women are more susceptible to oxidative damage and may develop pregnancy-associated complications.

Author Biographies

Niharika Singh, Department of Biochemistry, Integral Institute of Medical Sciences & Research (IIMS&R), Integral University, Lucknow, UP, India.

Saba Khan, Department of Biochemistry, Integral Institute of Medical Sciences & Research (IIMS&R), Integral University, Lucknow, UP, India.

Mohammad Mustufa Khan, Department of Basic Medical Sciences, Integral Institute of Allied Health Sciences & Research (IIAHS&R), Integral University, Lucknow, UP, India

Haseeb Ahsan, Department of Biochemistry, Faculty of Dentistry, Jamia Millia Islamia, Jamia Nagar, New Delhi, India

Roshan Alam, Department of Biochemistry, Integral Institute of Medical Sciences & Research (IIMS&R), Integral University, Lucknow, UP, India.

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