The effect of maja fruit (Aegle marmelos (L) correa) on inflammation markers involved in the aging process

  • David Limanan Department of Biochemistry and Molecular Biology, Faculty of Medicine, Tarumanagara University, Jakarta, Indonesia
  • NO Christian Department of Biochemistry and Molecular Biology, Faculty of Medicine, Tarumanagara University, Jakarta, Indonesia
  • E Efrany Department of Biochemistry and Molecular Biology, Faculty of Medicine, Tarumanagara University, Jakarta, Indonesia
  • E Yulianti Department of Biochemistry and Molecular Biology, Faculty of Medicine, Tarumanagara University, Jakarta, Indonesia
  • Frans Ferdinal Department of Biochemistry and Molecular Biology, Faculty of Medicine, Tarumanagara University, Jakarta, Indonesia
Keywords: aging, oxidative stress, tumor necrosis factor-alpha, interleukin-6, hypoxia

Abstract

Background: Aging is a process that every living creature will pass through and involves various factors. Increased inflammatory factors can be caused by hypoxia which forms Reactive Oxygen Species (ROS) and damages macromolecules and causes the acceleration of the aging process. Maja plants, empirically have long been used as medicinal plants, research on Maja leaves shows anti-inflammatory and antioxidant potential.

Objective: The purpose of this study was to find out and understand the potential of Maja fruit in the presence of inflammatory markers of IL-6 and TNF-α which are involved in the aging process.

Methods: This research was in vivo experimental, using male experimental animals Sprague Dawley rats divided into 8 groups (n = 4), and divided into 2 groups (fed with Maja ethanol extract (400 mg/ kg/day, 14 days) and not force-fed). Each group was divided into 4 subgroups (normoxia, hypoxia (O2 8%, N2 92%) for 3, 7 and 14 days). At the end of the trial period, animal blood was examined for IL-6 and TNF-α concentrations using the ELISA method.

Results: The results showed an increase in levels of IL-6 and TNF-α both in the group fed and not fed in line with the duration of hypoxia when compared with controls. However, groups that are not force-fed show a higher pattern compared to those who are force-fed.

Conclusion : The ethanol extract of Maja fruit can help slow down the aging process.

References

Kennely P. The Biochemistry of Aging. In: Rodwel V, Bender D, Botham K, Kennelly,Weil P. Harper's Illustrated Biochemistry. 30th Ed. McGrraw-Hill. United States. 2015. 755-67.

Bruunsgaard H, Skinhoj P, Pedersen N, Sschroll M, Pedersen K. Ageing, tumour necrosis factor-alpha (TNF-a) and atherosclerosis. Clin Exp Immunol. 2000;121:255-60.

https://doi.org/10.1046/j.1365-2249.2000.01281.x

Frijhoff J, Winyard P, Zarkovic N, Davies S, Stocker R, Cheng D. Clinical Relevance of Biomarkers of Oxidative Stress. Antioxidants and Redox Signaling. 2015;23(14):1144-70.

https://doi.org/10.1089/ars.2015.6317

Burton G, Jauniaux E. Oxidative stress. In : Arulkumaran S, Modder J (Ed). Best parctice and research clinical obstetrics and gynaecology. Elsevier. 2011;25:287 - 99.

Knuppel R, Hassan M, McDermott J, et al. Oxidative Stress and Antioxidants: Preterm Birth and Preterm Infants. In : Child John Morrison (Ed.). Preterm Birth - Mother and child. Intech. Croatia. 2012;125-50.

Gitto E, D'Angelo G, Cusumano E, Reiter R. Oxidative Stress of Newborn. In: Dr. Öner Özdemir (Ed.). Complementary Pediatrics. Intech. Croatia. 2012;73-96.

Dhankhar S, Ruhil S, Balhara M, Dhankar S, Chhillar A. Aegle Marmelos (Linn) Corre: A potential Source of Phytomedicine. J Med Plant Res. 2011;5(9):1497-507.

Rahman S, Parvin R. Therapeutic potential of Aegel Marmelos-An overview. Asian Pac J Trop Dis.2014;4(1):71-7.

https://doi.org/10.1016/S2222-1808(14)60318-2

Li SJ, Liu W, Wang JL, Zhang Y, Zhao DJ, Wang TJ, Li YY. The role of TNF-α, IL-6, IL-10, and GDNF in neuronal apoptosis in neonatal rat with hypoxic-ischemic encephalopathy. European Review for Medical and Pharmacological Sciences. 2014;18:905-9.

Bahera JP, Mohanty B, Ramani YR, Rath B, Pradhana S. Effect of aqueous extract of Aegle Marmelos unripe fruit on inflammatory bowel disease. Indian Journal of Pharmacology. 2012;44(5):614-8.

https://doi.org/10.4103/0253-7613.100389

Kuznicka M, Owczarz M, Tobis K, Nadrowski P, Chudek J. Interleuikin-6 and C-reactive protein successful aging, and mortality: the possenior study. Immunity and ageing. 2016:13(21):1-12.

https://doi.org/10.1186/s12979-016-0076-x

Sedger L, McDermott M. TNF and TNF-receptor: from mediators of cell death and inflammation to therapeutic giants. Cytokine and Growth Factor Review.2014:25(4):453-72.

https://doi.org/10.1016/j.cytogfr.2014.07.016

Arul V, Miyazaki S, Dhananjayan R (2005). Studies on the antiinflammatory, antipyretic and analgesic properties of the leaves of Aegle marmelos Correa. J. Ethnopharmacol., 96: 159.

https://doi.org/10.1016/j.jep.2004.09.013

Sedger L, McDermott M. TNF and TNF-receptor: from mediators of cell death and inflammation to therapeutic giants. Cytokine and Growth Factor Review.2014:25(4):453-72.

https://doi.org/10.1016/j.cytogfr.2014.07.016

Published
2018-06-30
How to Cite
Limanan, D., Christian, N., Efrany, E., Yulianti, E., & Ferdinal, F. (2018). The effect of maja fruit (Aegle marmelos (L) correa) on inflammation markers involved in the aging process. Acta Biochimica Indonesiana, 1(1), 24-30. https://doi.org/10.32889/actabioina.v1i1.4