Acute toxicity test of Rhizophora apiculata bark extract on rat liver and kidney histology using fixed dose method
Abstract
Background: The therapeutic properties of the ethanol extract derived from the stem bark of Rhizophora apiculata have been investigated. Nevertheless, there is a lack of studies regarding its acute toxicity.
Objective: This research aims to evaluate the toxicity of ethanolic extract of R. apiculata bark by examining histological changes in the liver and kidney of rats.
Method: The ethanol extract of R. apiculata bark was administered using a fixed-dose approach in preliminary and primary tests, focusing on the kidneys and liver of male Sprague Dawley rats. Four treatment groups received doses of 5, 50, 300, and 2000 mg/kg BW, while one group served as the control. An uninterrupted observation period of 14 days was conducted to determine any indications of acute toxicity in these animals. On the fifteenth day, the rats were terminated.
Results: This study indicated the absence of any toxic manifestations, such as tremors, excessive salivation, convulsions, coma, and mortality. Nevertheless, it caused histological damage to the rats’ liver and kidney when administered at doses 300 and 2000 mg/kg BW.
Conclusion: While the administration of R. apiculata extracts at doses of 300 and 2000 mg/kgBW resulted in histological damage to the kidneys and livers of the rats, it did not induce any immediate symptoms.
References
Rizki R, Leilani I. Etnofarmakologi tumbuhan familia Rhizophoraceae oleh masyarakat di Indonesia. J Bioconcetta. 2017;3(1):51-60. https://doi.org/10.22202/bc.2017.v3i1.2726
Nabeelah Bibi S, Fawzi MM, Gokhan Z, Rajesh J, Nadeem N, et al. Ethnopharmacology, phytochemistry, and global distribution of Mangroves-A comprehensive review. Mar Drugs. 2019;17(4):231. https://doi.org/10.3390/md17040231
Rahim AA, Rocca E, Steinmetz J, Jain Kassim M, Sani Ibrahim M, Osman H. Antioxidant activities of mangrove Rhizophora apiculata bark extracts. Food Chem. 2008 Mar 1;107(1):200-7. https://doi.org/10.1016/j.foodchem.2007.08.005
Gao M, Xiao H. Activity-guided isolation of antioxidant compounds from Rhizophora apiculata. Molecules. 2012;17(9):10675-82.https://doi.org/10.3390/molecules170910675
Thatoi HN, Patra JK, Das SK. Free radical scavenging and antioxidant potential of mangrove plants: A review. Acta Physiol Plant. 2014;36(3):561-79. https://doi.org/10.1007/s11738-013-1438-z
Mustofa S, Bahagia W, Kurniawaty E, Rahmanisa S, Audah KA. The effect of Mangrove (Rhizophora Apiculata) bark extract ethanol on histopathology pancreas of male white rats Sprague Dawley strain exposed to cigarette smoke. ActaBioIna. 2018;1(1):7-13. https://doi.org/10.32889/actabioina.v1i1.2
Mustofa S, Alfa N, Wulan AJ, Rahmanisa S. Pengaruh pemberian ekstrak kulit batang bakau minyak (Rhizophora apiculata) etanol 95% terhadap arteri koronaria tikus putih (Rattus novergicus) jantan galur Sprague Dawley yang dipaparkan asap rokok. J Kedokt Univ Lampung. 2019;3(1):28-33.
Mustofa S, Hanif F. The protective effect of rhizophora apiculata bark extract against testicular damage induced by cigarette smoke in male rats. ActaBiolna. 2019;2(1):23-31. https://doi.org/10.32889/actabioina.v2i1.34
Sulaiman S, Ibrahim D, Kassim J, Sheh-Hong L. Antimicrobial and antioxidant activities of condensed tannin from Rhizophora apiculata barks. J Chem Pharm Res. 2011;3(4):773-6.
Loo AY, Jain K, Darah I. Antioxidant and radical scavenging activities of the pyroligneous acid from a mangrove plant, Rhizophora apiculata. Food Chem. 2007 Jan 1;104(1):300-7. https://doi.org/10.1016/j.foodchem.2006.11.048
.Vinod Prabhu V, Guruvayoorappan C. Anti-inflammatory and anti-tumor activity of the marine mangrove Rhizophora apiculata. J Immunotoxicol. 2012;9(4):341-52. https://doi.org/10.3109/1547691X.2012.660997
Selvaraj G, Kaliamurthi S, Thirugnasambandan R. Effect of glycosin alkaloid from Rhizophora apiculata in non-insulin dependent diabetic rats and its mechanism of action: in vivo and in silico studies. Phytomedicine.2016; 23:632-40. https://doi.org/10.1016/j.phymed.2016.03.004
Mustofa, S., Ciptaningrum, I., & Zuya, C. S. (2020). Subacute toxicity test of Rhizophora apiculata bark extract on liver and pancreas histopathology of rats. Acta Biochimica Indonesiana, 3(2), 89-97. https://doi.org/10.32889/actabioina.v3i2.52
BPOM RI. Pedoman uji toksisitas nonklinik secara in vivo. Jakarta. Kepala Badan Pengawas Obat dan Makanan Republik Indonesia. 2014
Arndt TP, Boone LI. Clinical Pathology of the Rat. In: Suckow MA, Hankenson C, Wilson RP, Foley PL, editors. American College of Laboratory Animal Medicine,The Laboratory Rat (Third Edition). Academic Press; 2020.p. 133-155. https://doi.org/10.1016/B978-0-12-814338-4.00005-2
Chen J, Jiang S, Wang J, Renukuntla J, Sirimulla S, Chen J. A comprehensive review of cytochrome P450 2E1 for xenobiotic metabolism. Drug Met Rev. 2019: 51(2). https://doi.org/10.1080/03602532.2019.1632889
Klaunig JE, Li X, Wang Z. Role of xenobiotics in the induction and progression of fatty liver disease. Tox Res. 2018; 7(4): 664-80. https://doi.org/10.1039/c7tx00326a
Siriwardena AK. Reappraisal of xenobiotic-induced, oxidative stress-mediated cellular injury in chronic pancreatitis: a systematic review. World J Gastroenterol. 2014;20(11):3033-43. https://doi.org/10.3748/wjg.v20.i11.3033
Timotheo CA, Lauer CM. Toxicity of vegetable tannin extract from Acacia mearnsii in Saccharomyces cerevisiae . Int. J. Environ. Sci. Technol. 2018; 15: 659-64. https://doi.org/10.1007/s13762-017-1430-z
Mahmud KN, Hashim NM, Ani FN, et al. Antioxidants, Toxicity, and Nitric Oxide Inhibition Properties of Pyroligneous Acid from Palm Kernel Shell Biomass. Waste Biomass Valor. 2019. https://doi.org/10.1007/s12649-019-00857-w
Puteri FH, Widjaja J, Cahyani F, Mooduto L, Wahjuningrum DA. The Comparative Toxicity of Xanthones and Tannins in Mangosteen (Garcinia mangostana Linn.) Pericarp Extract against BHK-21 Fibroblast Cell Culture. Contemp Clin Dent. 2019;10(2):319-23. https://doi.org/10.4103/ccd.ccd_579_18
Smeriglio A, Barreca D, Bellocco E, Trombetta D. Proanthocyanidins and hydrolysable tannins: occurrence, dietary intake and pharmacological effects. Br J Pharmacol. 2017;174(11):1244-62. https://doi.org/10.1111/bph.13630
Liu Z, Zhou L, Liu D, Zhu Q, Chen W. Inhibitory mechanisms of Acacia mearnsii extracts on the growth of Microcystis aeruginosa. Water Sci Technol. 2015; 71(6):856-61. https://doi.org/10.2166/wst.2015.038
Scharf, P., Rizzetto, F., Xavier, L. F., & Farsky, S. H. P. (2022). Xenobiotics Delivered by Electronic Nicotine Delivery Systems: Potential Cellular and Molecular Mechanisms on the Pathogenesis of Chronic Kidney Disease. International Journal of Molecular Sciences, 23(18), 10293. https://doi.org/10.3390/ijms231810293
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