The correlation between plasma malondialdehyde levels and pain in adolescent females diagnosed with primary dysmenorrhea
Background: Endometrial inflammation factors play a crucial role in the common pathophysiology of primary dysmenorrhea. Malondialdehyde (MDA), a byproduct of inflammatory processes, might be correlated with primary dysmenorrhea in adolescents.
Objective: To assess plasma MDA levels in patients with primary dysmenorrhea.
Methods: This cross-sectional study involved the collection of blood samples from 23 teenage females with primary dysmenorrhea and 23 age-matched individuals without this condition. Plasma MDA levels were quantified through spectrophotometry. The independent t-test was employed to ascertain the disparity in plasma MDA levels between the two groups, while the Pearson correlation test was utilized to analyze the relationship between pain severity and oxidative stress levels.
Results: Plasma MDA levels were significantly higher in females with primary dysmenorrhea than in the control group (0.631 ± 0.105 and 0.395 ± 0.076, respectively). A significant difference in oxidative stress levels was observed between the case and control groups (p<0.001). Furthermore, a robust positive correlation was observed between plasma MDA levels and pain severity in adolescent females with dysmenorrhea (r2 = 0.564, p<0.001).
Conclusion: The plasma MDA is increased in adolescent females with primary dysmenorrhea.
Hassan N, Moien AK. Dysmenorrhea, https://pubmed.ncbi.nlm.nih.gov/32809669/#affiliation-1 (2022, accessed 20 August 2022).
Harel Z. Dysmenorrhea in adolescents and young adults: etiology and management. J Pediatr Adolesc Gynecol 2006; 19: 363-371. https://doi.org/10.1016/j.jpag.2006.09.001
Sachedina A, Todd N. Dysmenorrhea, endometriosis and chronic pelvic pain in adolescents. JCRPE Journal of Clinical Research in Pediatric Endocrinology 2020; 12: 7-17. https://doi.org/10.4274/jcrpe.galenos.2019.2019.S0217
Gutman G, Nunez AT, Fisher M. Dysmenorrhea in adolescents. Curr Probl Pediatr Adolesc Health Care; 52. Epub ahead of print 1 May 2022. https://doi.org/10.1016/j.cppeds.2022.101186
Sanfilippo J, Erb T. Evaluation and management of dysmenorrhea in adolescents. Clin Obstet Gynecol 2008; 51: 257-267. https://doi.org/10.1097/GRF.0b013e31816d2307
Sahin N, Kasap B, Kirli U, et al. Assessment of anxiety-depression levels and perceptions of quality of life in adolescents with dysmenorrhea. Reprod Health; 15. Epub ahead of print 26 January 2018. https://doi.org/10.1186/s12978-018-0453-3
Greydanus DE, McAnarney ER. Menstruation and its disorders in adolescence. Curr Probl Pediatr 1982; 12: 6-61. https://doi.org/10.1016/0045-9380(82)90034-2
Muzii L, Catalano GF, Marana R, et al. Correlation between endometriosis-associated dysmenorrhea and the presence of typical or atypical lesions. Fertil Steril 1997; 68: 19-22.
Turhan N, Çelik H, Duvan CI, et al. Investigation of oxidative balance in patients with dysmenorrhea by multiple serum markers. J Turk Ger Gynecol Assoc 2012; 13: 233. https://doi.org/10.5152/jtgga.2012.36
Itani R, Soubra L, Karout S, et al. Primary Dysmenorrhea: Pathophysiology, Diagnosis, and Treatment Updates. Korean J Fam Med 2022; 43: 101-108. https://doi.org/10.4082/kjfm.21.0103
Strömberg P, Åkerlund M, Forsling ML, et al. Vasopressin and prostaglandins in premenstrual pain and primary dysmenorrhea. Acta Obstet Gynecol Scand 1984; 63: 533-538.
Andrew S, Coco M. Primary dysmenorrhea. Am Fam Physician 1999; 60: 489-96.
Liedman R, Hansson SR, Howe D, et al. Reproductive hormones in plasma over the menstrual cycle in primary dysmenorrhea compared with healthy subjects. Gynecol Endocrinol 2008; 24: 508-513. https://doi.org/10.1080/09513590802306218
Evans MD, Dizdaroglu M, Cooke MS. Oxidative DNA damage and disease: Induction, repair and significance. Mutat Res Rev Mutat Res 2004; 567: 1-61. https://doi.org/10.1016/j.mrrev.2003.11.001
Rao V, R K, M V. Oxidative stress and antioxidant status in primary dysmenorrhea. Journal of clinical and diagnostic research 2011; 5: 509-11.
Irianti B, Ermawati E, Amir A. Perbedaan Kadar Malondialdehide dan Tromboksan B2 pada Remaja dengan Dismenore dan Tanpa Dismenore. Jurnal Kesehatan Andalas; 4. Epub ahead of print 1 September 2015. https://doi.org/10.25077/jka.v4i3.350
Niki E. Lipid peroxidation: Physiological levels and dual biological effects. Free Radic Biol Med 2009; 47: 469-484. https://doi.org/10.1016/j.freeradbiomed.2009.05.032
Be O, Awolude OA, Agbedana EO. Markers of Lipid and Protein Peroxidation among Nigerian University Students with Dysmenorrhea. Niger J Clin Pract 2019; 22: 147-180.
Ocktariyana O, Wahyuni S. Role of natural antioxidant after exercise in reducing malondialdehyde (MDA) levels during pregnancy. J Pak Med Assoc 2021; 71(Suppl 2): S14-S17.
Francesca F, Bader P, Echtle D, et al. Guidelines on Pain Management. European Association of Urology, 2007.
Ocktariyana, Hikmawati N, Hestiantoro A, et al. Analysis of DNA methylation level and mRNA expression of Transient Receptor Ankyrin Member 1 (TRPA1) in endometriosis-associated pain. AsPac J Mol Biol Biotechnol 2021; 29: 1-10. https://doi.org/10.35118/apjmbb.2021.029.3.01
Kaplan Ö, Naziroǧlu M, Güney M, et al. Non-steroidal anti-inflammatory drug modulates oxidative stress and calcium ion levels in the neutrophils of patients with primary dysmenorrhea. J Reprod Immunol 2013; 100: 87-92. https://doi.org/10.1016/j.jri.2013.10.004
Dikensoy E, Balat O, Pençe S, et al. Malondialdehyde, nitric oxide and adrenomedullin levels in patients with primary dysmenorrhea. Journal of Obstetrics and Gynaecology Research 2008; 34: 1049-1053. https://doi.org/10.1111/j.1447-0756.2008.00802.x
Wei Y, Ma T, Wang H, et al. Extracts of compound Muniziqi granule suppressed uterus contraction and ameliorated oxytocin-induced primary dysmenorrhea. J Ethnopharmacol 2018; 223: 33-40. https://doi.org/10.1016/j.jep.2018.05.024
Szmidt MK, Granda D, Sicinska E, et al. Primary Dysmenorrhea in Relation to Oxidative Stress and Antioxidant Status: A Systematic Review of Case-Control Studies. Antioxidants 2020, Vol 9, Page 994 2020; 9: 994. https://doi.org/10.3390/antiox9100994
Higashi Y, Maruhashi T, Noma K, et al. Oxidative stress and endothelial dysfunction: Clinical evidence and therapeutic implications. Trends Cardiovasc Med 2014; 24: 165-169.
Orimadegun B, Awolude O, Agbedana E. Markers of lipid and protein peroxidation among Nigerian university students with dysmenorrhea. Niger J Clin Pract 2019; 22: 174-180.
Bonnes-Taourel D, Guérin MC, Torreilles J. Is malonaldehyde a valuable indicator of lipid peroxidation? Biochem Pharmacol 1992; 44: 985-988. https://doi.org/10.1016/0006-2952(92)90132-3
Balat O, Dikensoy E, Ugur MG, et al. Malon dialdehyde, nitrite and adrenomedullin levels in patients with premenstrual syndrome. Arch Gynecol Obstet 2007; 275: 361-365. https://doi.org/10.1007/s00404-006-0269-1
Yeh ML, Chen HH, So EC, et al. A study of serum malondialdehyde and interleukin-6 levels in young women with dysmenorrhea in Taiwan. Life Sci 2004; 75: 669-673. https://doi.org/10.1016/j.lfs.2003.11.034
Kazemi M, Lalooha F, Nooshabadi MR, et al. Decreased dysmenorrhea pain in girls by reducing oxidative stress and inflammatory biomarkers following supplementation with oleoylethanolamide: A randomized controlled trial. J Obstet Gynaecol Res 2022; 48: 1212-1221. https://doi.org/10.1111/jog.15196
Kilic N, Yavuz Taslipinar M, Guney Y, et al. An Investigation into the Serum Thioredoxin, Superoxide Dismutase, Malondialdehyde, and Advanced Oxidation Protein Products in Patients with Breast Cancer. Annals of Surgical Oncology 2014 21:13 2014; 21: 4139-4143. https://doi.org/10.1245/s10434-014-3859-3
Seraj AK, Shankhar M, Raju KD, et al. Antioxidants and Lipid Peroxidation Status In Women with Breast Cancer. IIUM Medical Journal Malaysia 2015; 14: 71-75. https://doi.org/10.31436/imjm.v14i1.459
Gupta RK, Patel AK, Kumari R, et al. Interactions between Oxidative Stress, Lipid Profile and Antioxidants in Breast Cancer: A Case Control Study. Asian Pacific Journal of Cancer Prevention 2012; 13: 6295-6298. https://doi.org/10.7314/APJCP.2012.13.12.6295
Jelic MD, Mandic AD, Maricic SM, et al. Oxidative stress and its role in cancer. J Cancer Res Ther 2021; 17: 22. https://doi.org/10.4103/jcrt.JCRT_862_16
Lucas BS, Navdeep SC. Mitochondrial reactive oxygen species and cancer. Cancer Metab 2014; 2: 2-12. https://doi.org/10.1186/2049-3002-2-17
Ocktariyana. Nyeri pada Endometriosis dalam Perspektif Molekuler. 1st ed. Kediri, Jawa Timur: Chakra Brahmanda Lentera, https://www.researchgate.net/profile/Ocktariyana-Ocktariyana/publication/357518032_Nyeri_Pada_Endometriosis_Dalam_Perspektif_Molekuler/links/61d23cfdb6b5667157c2e96f/Nyeri-Pada-Endometriosis-Dalam-Perspektif-Molekuler.pdf (2020, accessed 19 October 2022).
Copyright (c) 2023 Authors
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.