Jamblang (Syzygium cumini) leaf extract decreased hydrogen peroxide in lead acetate-induced rats
Abstract
Background: Free radicals are atoms or molecules with one or more unpaired electrons. Lead acetate has been reported to increase the presence of free radicals in the body.
Objective: This study aims to investigate the effect of oral administration of jamblang (Syzygium cumini) leaf extract on hydrogen peroxide (H2O2) serum levels in rats induced with lead acetate.
Methods: A total of 24 male rats were divided into three groups: the negative control, the positive control, and the treatment group. The negative control group received a standard diet, the positive control group received lead acetate at a dose of 40 mg/kg body weight, and the treatment group received lead acetate at the same dose along with jamblang leaf extract at a dose of 150 mg/kg body weight. Lead acetate and jamblang leaf extract administration was carried out for 30 days. Afterward, serum H2O2 levels were examined using the colorimetry method.
Results: Results revealed that H2O2 levels in the negative control, positive control, and treatment groups were 3.08±0.24, 4.94±0.75, and 3.44±0.65 nmol/L, respectively. Significant differences were observed between the groups, as well as between the negative control and positive control, and between the positive control and treatment group.
Conclusion: The study showed that jamblang leaf extract can reduce hydrogen peroxide levels in Wistar rats treated with lead acetate, indicating its ability to address oxidative stress.
References
Rădulescu A, Lundgren S. A pharmacokinetic model of lead absorption and calcium competitive dynamics. Sci Rep. 2019;9. https://doi.org/10.1038/s41598-019-50654-7
Debnath B, Singh W, Manna K. Sources and toxicological effects of lead on human health. Indian J Med Spec. 2019;10:66-71. https://doi.org/10.4103/INJMS.INJMS_30_18
Jaishankar M, Tseten T, Anbalagan N, Mathew BB, Beeregowda KN. Toxicity, mechanism and health effects of some heavy metals. Interdiscip Toxicol. 2014;7:60-72. https://doi.org/10.2478/intox-2014-0009
Patra RC, Rautray AK, Swarup D. Oxidative stress in lead and cadmium toxicity and its amelioration. Vet Med Int. 2011. https://doi.org/10.4061/2011/457327
Jan AT, Azam M, Siddiqui K, Ali A, Choi I, Haq QMR. Heavy metals and human health: Mechanistic insight into toxicity and counter defense system of antioxidants. Int J Mol Sci. 2015;16:29592-630. https://doi.org/10.3390/ijms161226183
Zenin V, Ivanova J, Pugovkina N, Shatrova A, Aksenov N, Tyuryaeva I, et al. Resistance to H2O2-induced oxidative stress in human cells of different phenotypes. Redox Biol. 2022;50. https://doi.org/10.1016/j.redox.2022.102245
Ni Z, Hou S, Barton CH, Vaziri ND. Lead exposure raises superoxide and hydrogen peroxide in human endothelial and vascular smooth muscle cells. Kidney Int. 2004;66:2329-36. https://doi.org/10.1111/j.1523-1755.2004.66032.x
Espinosa-Diez C, Miguel V, Mennerich D, Kietzmann T, Sánchez-Pérez P, Cadenas S, et al. Antioxidant responses and cellular adjustments to oxidative stress. Redox Biol. 2015;6:183-197. https://doi.org/10.1016/j.redox.2015.07.008
Hasanuzzaman M, Bhuyan MHMB, Zulfiqar F, Raza A, Mohsin SM, al Mahmud J, et al. Reactive oxygen species and antioxidant defense in plants under abiotic stress: Revisiting the crucial role of a universal defense regulator. Antiox. 2020;9:1-52. https://doi.org/10.3390/antiox9080681
Rahayu SE, Dharmawan A, Putri VAL. Potensi ekstrak daun jamblang (Syzigium cumini L.) sebagai larvasida untuk pengendalian larva nyamuk Aedes aegypti. Jurnal Ilmiah Biosaintropis (Bioscience-Tropic). 2021;6:26-33. https://doi.org/10.33474/e-jbst.v6i2.380
Rita RS, Sy E. Syzygium cumini leaves extract from West Sumatra Indonesia alleviate oxidative stress by decreasing malondialdehyde level and enhancing catalase activity in rat induced by lead acetate. Phcog J. 2021;13:1408-12. https://doi.org/10.5530/pj.2021.13.178
Sari AN, Biologi P, Sains F, Uin T, Raniry A, Aceh B. Potensi antioksidan alami pada ekstrak daun jamblang (Syzigium cumini (L.) Skeels). Eksakta. 2017;18:107-112. https://doi.org/10.24036/eksakta/vol18-iss02/61
Flora G, Gupta D, Tiwari A. Toxicity of lead: A review with recent updates. Interdisciplinary Toxicology. 2012 47-58. https://doi.org/10.2478/v10102-012-0009-2
Dumanović J, Nepovimova E, Natić M, Kuča K, Jaćević V. The Significance of Reactive Oxygen Species and Antioxidant Defense System in Plants: A Concise Overview. Front Plant Sci. 2021;11:1-13. https://doi.org/10.3389/fpls.2020.552969
Zeng Y, Song J, Zhang M, Wang H, Zhang Y, Suo H. Comparison of in vitro and in vivo antioxidant activities of six flavonoids with similar structures. Antioxidants. 2020;9:1-14. https://doi.org/10.3390/antiox9080732
Ahmed R, Tariq M, Hussain M, Andleeb A, Masoud MS, Ali I, et al. Phenolic contents-based assessment of therapeutic potential of Syzygium cumini leaves extract. PLoS One. 2019;14(8). https://doi.org/10.1371/journal.pone.0221318
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