Malondialdehyde and carbonyl levels in skeletal muscle tissues after intermittent hypobaric hypoxia exposures
Abstract
Background: Hypobaric hypoxia is a hypoxic condition in high altitude because of low oxygen pressure, which can induce oxidative stress. Skeletal muscle is one of important organ that could be affected by oxidative stress and cause contractile dysfunction.
Objective: The aim of this study was investigating the effect of intermittent hypobaric hypoxia induction on malondialdehyde (MDA) and carbonyl levels as oxidative stress marker in rat skeletal muscle.
Methods: This is an in vivo experimental study that carried out by conditioning four groups of Wistar rats in a hypobaric hypoxic state in a hypobaric chamber one- (I), two- (II), three- (III), and four- (IV) times (IV) with interval 7 days. Rats in normobaric conditions act as control group. The gastrocnemius muscle of all rats was taken to measure the MDA and carbonyl level.
Results: The MDA level was significantly increased in the group I compared to control group (p<0.05). There were decreasing of MDA level in group II, III and IV compared to group I. The carbonyl level was found significantly increased in group I compared to control group (p<0.05), but the higher level was found in group II. Carbonyl level tend to decrease in group III and IV, but the level was still higher compared to control group.
Conclusion: We concluded that the MDA and carbonyl levels were increased in skeletal muscle after one-time hypobaric hypoxia exposure which meant oxidative stress increases, and they tend to decrease after intermittent hypobaric hypoxia exposure which indicate an adaptation of skeletal muscle to hypoxic condition.
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