Syzygium aromaticum (clove) effect on catalase activity due to carbon tetrachloride-induced oxidative stress in rat liver

  • Ani Retno Prijanti Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
  • AA Hawali Medical Program, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
Keywords: clove, carbon tetrachloride, catalase, liver, vitamin e

Abstract

Background: Clove is known as antioxidant spice that used in cigarettes, spice for food or soup, and traditional medicine. It is believed that cloves could protect smokers from cigarette-free radicals. Otherwise, the study on clove as an antioxidant was still confused.

Objective: To reveal that clove can overcome carbon tetrachloride (CCl4) and its free-radical derives

Method:  This study was experimental research, using 20 Wistar rats that were divided into 4 groups, Group 1 (CCl4 + cloves 3), group 2 (CCl4 + cloves 1), group 3 (normal control, without being offered treatment), group 4 (positive control, induced by CCl4 and followed by 100 mg alpha-tocopherol), and group 5 (negative control, only induced by CCl4). Rat livers were homogenized and followed with CAT activity measurement using the spectrophotometry method of Mates.

Results: There was a significant difference in mean between the groups (p= 0,001). Further test, the Post Hoc showed that there is a significance different between group 1 and 4 (p=0.008), 1 and 5 (p=0.001), 2 and 5 (p=0.001), 3 and 5 (p=0.001), and 4 and 5 (p=0.007). Group 1 (CCl4+Clove3) has the highest catalase activity.

Conclusion: Syzygium aromaticum (clove) oral administration with the dose of 200 mg/kg rat body weight against 0.55 mg/kg BW CCl4 show an increase of catalase activity but did not overcome the oxidative stress.

References

Murray RK, Granner DK, Rodwell VW. Harper's Illustrated Biochemistry. 28th ed. Boston: McGraw Hill; 2009.

Agency for Toxic Substances and Disease Registry [internet]. Carbon tetrachloride (accessed 2015 April 30). Available from : http://www.atsdr.cdc.gov/substances/toxsubstance.asp?toxid=35.

United States Environmental Protection Agency [internet]. Carbon tetrachloride (accessed 2015 April 30). Available from: http://www.epa.gov/ttn/atw/hlthef/carbonte.html#ref1.

Kuntz E, Kuntz HD. Hepatology Principles and Practice. 2nd Ed. Berlin: Springer Medizin Verlag; 2006.

https://doi.org/10.1007/3-540-28977-1

Halliwell B, Gutteridge JM. Free radicals in Biology and Medicine. 4th ed. New York : Oxford University Press; 2007.

Lawless. The illustrated Encyclopedia of Essential Oils. J; 1995.

Tiwari P, Verma R, Ahirwar D, Chandy A, Dwivedi S. Evaluation of anxiolytic effect of syzygium aromatic: a traditional herb of India. Asian Pasific Journal of Tropical Disease. 2014; 4 : 77-80.

https://doi.org/10.1016/S2222-1808(14)60418-7

Rojas DFC, Souza CRF, Oliveira WP. Clove (Syzygium aromaticum): A precious spice. Asian Pac J Trop Biomed. 2014; 4(2): 90-96.

https://doi.org/10.1016/S2221-1691(14)60215-X

Nurdjannah N. Diversifikasi Penggunaan Cengkeh. Indonesian Center for Agricultural Postharvest Research and Development; 2004.

Devi KP, Nisha SA, Sakthivel R, Pandian SK. Eugenol (an essential oil of clove) acts as an antibacterial agent against Salmonella typhi by disrupting the cellular membrane. J Ethnopharmacol. 2010; 130(1): 107-15.

https://doi.org/10.1016/j.jep.2010.04.025

Rojas DFC, Souza CRF, Oliveira WP. Clove (Syzygium aromaticum): A precious spice. Asian Pac J Trop Biomed. 2014; 4(2): 90-96.

https://doi.org/10.1016/S2221-1691(14)60215-X

Azwar MK, Prijanti AR. Clove (Syzygium aromaticum) effect on Carbon tetra cloride-induced rats: Comparison of malondialdehyde level of liver and blood plasma. Advance Science Letters. 2018; 24(9): 6976-79.

https://doi.org/10.1166/asl.2018.12620

Kusmawanto. Comparrison between Liver and plasma Carbonyl compound concentration in CCl4 induced rat treated with time bound clove extract [Thesis]. 2017.

Sobri M. Efek Cengkeh pada Tikus yang di Induksi CCl4 : Perubahan kadar Glutation pada organ hati dan jantung [Thesis]. 2018.

Karp G. Cell and Molecular Biology: Concepts and Experiments. 7th ed. Hoboken, NJ: Wiley; 2013.

Kang K, Lee K, Chae S, Zhang R, Jung M, Ham, et al. Cytoprotective effect of phloroglucinol on oxidative stress induced cell damage via catalase activation. J. Cell. Biochem. 2006 ; 97(3) : 609-620.

https://doi.org/10.1002/jcb.20668

Mhamdi A, Queval G, Chaouch S, Vanderauwera S, Van BF, Noctor G. Catalase function in plants: a focus on Arabidopsis mutants as stress-mimic models. Journal of Experimental Botany. 2010; 61(15): 4197-4220.

https://doi.org/10.1093/jxb/erq282

Mikalsen S, Kaalhus O, Reith A, Sanner T. Role of catalase and oxidative stress in hepatic peroxisome proliferator-induced morphological transformation of syrian hamster embryo cells. International Journal of Cancer. 1990; 46(5): 950-957.

https://doi.org/10.1002/ijc.2910460533

Mocchegiani E, Costarelli L, Giacconi R, Malavolta M, Basso A, Piacenza F,et al. Vitamin E-gene interactions in aging and inflammatory age-related diseases: Implications for treatment. A systematic review. Ageing Research Reviews. 2014:81-101.

https://doi.org/10.1016/j.arr.2014.01.001

Published
2018-06-30
How to Cite
Prijanti, A. R., & Hawali, A. (2018). Syzygium aromaticum (clove) effect on catalase activity due to carbon tetrachloride-induced oxidative stress in rat liver. Acta Biochimica Indonesiana, 1(1), 31-36. https://doi.org/10.32889/actabioina.v1i1.5